Figure 3.

Determination of the Shewanella lipBA as an operon. (A) Genetic organization and transcriptional analyses of Shewanella lipBA operon. The three arrows represent ybeD (in gray), lipB (in yellow) and lipA (in orange), respectively. The numbered short lines (1, 2, 3, 4, and 5) indicate the specific PCR amplicons. The transcription start sites (S) is indicated with an arrow. The PCR and RT-PCR products were separated by the electrophoresis of 1.5% agarose gel. (B) Electrophoretic analyses for the 5′-RACE product of Shewanella lipBA operon 5′-RACE product were separated with 2.0% agarose gel and the expected size was highlighted with an arrow. kb, kilo-base pair; M, 100 bp DNA ladder (New England Bio-labs, Ipswich, MA, USA). RT-PCR, reverse transcription-polymersase chain reaction.