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. 2015 Jan 22;4(2):282–300. doi: 10.1002/mbo3.237

Figure 4.

Figure 4

Use of 5′-RACE analyses to map the Shewanella lipBA promoter. (A) Direct DNA sequencing of the RLM-RACE product of the Shewanella lipBA operon. (B) Sequence comparison of the promoter regions of the Shewanella lipBA operon. The multiple alignments were conducted using ClustalW2 (http://www.ebi.ac.uk/Tools/clustalw2/index.html), and the resultant output was processed by program ESPript 2.2 (http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi) (Feng and Cronan 2009b, 2010; Feng et al. 2013a). Identical residues are indicated with white letters on a red background, similar residues are red letters on yellow, varied residues are in black letters, and dots represent missing residues. S, transcription start site; M, translational initiation site. The predicted CRP-recognizable palindrome is underlined. CRP, cAMP-receptor protein.