Table 1.
Bacterial strains and plasmids in this study
| Bacteria or plasmids | Relevant characteristics | Refs or origins |
|---|---|---|
| Bacterial strains | ||
| Escherichia coli | ||
| BL21(DE3) | Engineered E. coli strain as an expression host for recombinant plasmids | Lab stock |
| MG1655 | Wild type of E. coli K-12 (F-, λ-, rph-1) | CGSC1, Lab stock |
| WM3064 | Donor strain for conjugation; ΔdapA | W. Metcalf, UIUC |
| BW25113 | A Δlac strain of E. coli K-12 (F-, λ-, rph-1, Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) Δ(rhaD-rhaB)568 hsdR514) | CGSC1, Baba et al. (2006) |
| JW5702-4 | (BW25113, Δcrp-765::kan) | CGSC1, Baba et al. (2006), Feng and Cronan (2010) |
| MC1061 | F-, λ-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2 | Lab stock, Casadaban and Cohen (1980), Feng and Cronan (2009b) |
| RH77 | MC4100, ΔcyaA, Δcrp::Tn10 | Lab stock Feng and Cronan (2010, 2012) |
| DH5α (λ-pir) | An E. coli Δlac host for pAH125 and its derivatives | Feng and Cronan (2009a, 2012), Haldimann and Wanner (2001) |
| FYJ208 | Vibrio cholerae O395 | Jame Jun Zhu's lab |
| FYJ239 | BL21(DE3) carrying pET28-crp_ec | Feng and Cronan (2012), Goble et al. (2013) |
| FYJ426 | Salmonella enterica serovar Typhimurium 14028s | Slauch's lab |
| FYJ452 | DH5a(λ-pir) carrying pAH-PlipBA_she | This work |
| FYJ453 | MC4100 whose chromosome was integrated with the lipBA_she-lacZ transcriptional fusion at the λ phage site | This work |
| FYJ457 | MC1061, lipBA_she-lacZ transcriptional fusion | P1vir(FYJ453) × MC10612, This work |
| FYJ458 | MC4100, ΔcyaA, Δcrp::Tn10, lipBA_she-lacZ transcriptional fusion | P1vir(FYJ453) × RH772, This work |
| FYJ462 | Topo carrying pET28-crp_she | This work |
| FYJ463 | BL21(tuner) carrying pET28-crp_she | This work |
| S. oneidensis | ||
| MR-1 | Wild-type | Gao's lab |
| HG0624 | Δcrp derived from MR-1 | Gao et al. (2010) |
| HG1162-1 | ΔlipBA derived from MR-1 | This work |
| HG0424 | ΔaceE derived from MR-1 | This work |
| HG1329 | ΔcyaC derived from MR-1 | This work |
| Plasmids | ||
| pET28(a) | Commercial T7-driven expression vector, KmR | Novagen |
| pET28-crp_ec | pET28(a) carrying E. coli crp gene, KmR | Feng and Cronan (2012), Goble et al. (2013) |
| pAH125 | A promoter-less lacZ reporter plasmid in E. coli, KmR | Haldimann and Wanner (2001) |
| pHG101 | A promoter-less broad-host KmR vector | Wu et al. (2011) |
| pHGEI01 | An integrative lacZ reporter vector | Fu et al. (2014) |
| pAH-PlipBA_she | A pAH125 derivative encoding Shewanella oneidensis lipBA promoter region (∽350 bp) | This work |
| pET28-crp_she | pET28(a) encoding S. oneidensis crp gene, KmR | This work |
1
CGSC denotes Coli Genetic Stock Center, Yale University.
2
Selection for kanamycin resistance.