Table 2.
Estimates of taxon richness and diversity indices for 16S rRNA gene-454-pyrosequencing and the clone library based on various evolutionary distance criteria for demarcating operational taxonomic units
| Evolutionary distance | Richness | Diversity index | ||||
|---|---|---|---|---|---|---|
| No. of OTUs | ACE | Boot | Chao1 | Shannon | Simpson | |
| Pyro seqs | ||||||
| 0.03 | 12,621 | 23,489 | 14,920 | 19,871 | 7.75 | 526 |
| 0.05 | 7500 | 10,718 | 8687 | 10,833 | 7.03 | 307 |
| 0.1 | 3126 | 4060 | 3535 | 4096 | 5.77 | 79 |
| 0.2 | 807 | 1016 | 899 | 1024 | 4.02 | 14 |
| Clones | ||||||
| 0.03 | 73 | 199 | 92 | 189 | 3.95 | 48 |
| 0.05 | 60 | 165 | 75 | 130 | 3.52 | 21 |
| 0.1 | 40 | 80 | 49 | 69 | 2.72 | 7 |
| 0.2 | 18 | 29 | 21 | 28 | 1.64 | 3 |
OTU, operational taxonomic unit; ACE, abundance-based coverage estimator.