Figure 4.

Suppressor mutants isolated from the chimera strains. (A) Swimming abilities in soft agar plates of chimeras in Escherichia coli were examined. Fresh colonies of RP6894 (E. coli ΔmotAB), which were retransformed with the plasmids recovered from the suppressor strains, were inoculated and incubated on 0.3% TB agar for 22–24 h at 30°C with 0.02% arabinose. 1, vector plasmid pBAD33 (Vec); 2, pTF9 (PotB₅₉); 3, pTSK62 (PotB₉₁); 4, PotB₉₁-sp1; 5, pTSK64 (PotB₁₃₈); 6, PotB₁₃₈-sp1; 7, PotB₁₃₈-sp2; 8, PotB₁₃₈-sp3. (B) The mutation sites of the suppressors mapped on a topology model of PomA and PomB.