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. 2015 Jan 5;593(Pt 4):871–886. doi: 10.1113/jphysiol.2014.286633

Figure 3. The effects of JWH133 on GABAergic transmission and tests for the specificity of CB2R drugs.

Figure 3

A, rat hippocampal slice cultures were treated with JWH133 (200 nm) for 7–10 days, and miniature inhibitory postsynaptic currents (mIPSCs) were recorded from CA1 pyramidal neurons. B, evoked inhibitory postsynaptic currents (eIPSCs) were recorded from CA1 pyramidal neurons in rat hippocampal slice cultures, and JWH133 (200 nm), a CB2R agonist, was applied to the bath solution. Before the JWH133 application, postsynaptic cells were depolarized to 0 mV for 5 s to induce DSI, which is CB1R-mediated suppression of IPSCs. The eIPSC trace for DSI is an average of the first two eIPSCs after depolarization. C, DSI elicited by postsynaptic depolarization to 0 mV for 5 s was measured before and during bath application of SR144528 (200 nm; 10–20 min) or AM251 (300 nm; 7–11 min). Two eIPSCs after depolarization were averaged for the DSI traces. Error bars indicate SEM.