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. 2015 Jan 26;593(Pt 4):905–927. doi: 10.1113/jphysiol.2014.283374

Figure 1. Expression profile of Nav channel α and β-subunit genes and immunodetection of Nav1.3 and Nav1.7 proteins in the mouse adrenal medulla.

Figure 1

A, RT-qPCR data illustrating that, among all Nav channel genes, only TTX-sensitive isoforms are expressed in the adrenal medulla, with major expression for mRNAs encoding Nav1.3 and Nav1.7 (left: ***P < 0.001 for scn3a compared to scn9a). Except for scn4b (β4-subunit), all β-subunit mRNAs are present (right: **P < 0.01 for scn3b compared to scn1b). Significance tests between groups of data were performed using one-way ANOVA followed by a post hoc Bonferroni test. Data are the mean ± SEM of six independent samples. B, western blot analysis of proteins extracted from macrodissected mouse adrenal medulla (am, 10 μg) and from mouse brain (b, 30 μg). Note that a 3-fold higher amount of proteins was used for the brain as a result of the weak expression of Nav1.3 and Nav1.7 channels, as reported previously (Sangameswaran et al. 1997; Catterall et al. 2005). When probed for Nav1.3 and Nav1.7 proteins, a single band is immunodetected at 230 kDa (Nav1.3) and 220 kDa (Nav1.7) in both tissues, with stronger signal intensities in the adrenal medulla (upper membranes). Immunoblotting of β-actin was used as a control (lower membranes).