Table 1.
Sequence of primers used for quantitative RT-PCR
| Gene name | GenBank accession number | Protein name | forward primer (5′ to 3′) | reverse primer (5′ to 3′) |
|---|---|---|---|---|
| scn1A | NM_018733.2 | Nav1.1 | TTGCAAGGGGCTTCTGTTTA | AGGTCCACAAACTCCGTCAC |
| scn2A | NM_001099298.2 | Nav1.2 | GGGTTGCATATGGTTTCCAA | CCCAAGGCATTTGCAGTTA |
| scn3A | NM_018732.3 | Nav1.3 | TCCTCAGTAGTGGTGCTTTGG | GATGTAAGTGAAGACTTTGTCAGCA |
| scn4A | NM_133199.2 | Nav1.4 | GAAAACCATCACGGTCATCC | TCCGAGAGCTTTTTCACAGAC |
| scn5A | NM_021544.4 | Nav1.5 | GCCAGATCTCTATGGCAACC | TTGCCCTTATTCAGCACGAT |
| scn8A | NM_001077499.2 | Nav1.6 | CTGGTGCTGGTTGGACTTC | GCCCAGGGCATTAGCTATAA |
| scn9A | NM_018852.2 | Nav1.7 | AAAGCAGGTGGGACAAAGG | CTCTCCTTGGCACTCTTTGAG |
| scn10a | NM_009134.3 | Nav1.8 | GCTGAGCCTATCAATGCAGA | ACTTGGCAGCATGGAAATCT |
| scn11A | NM_011887.3 | Nav1.9 | TGGGTAGCTTATGGCTTCAAA | CTATGAGGCTTGTGAGGGAGA |
| scn1B | NM_011322.2 | β1 | TGCTACAAGAAGATTGCTGCTG | AATGGCCAGGTATTCTGAGG |
| scn2B | NM_001014761.2 | β2 | CAGGAGTGTAACAATTGCACAGA | GGGTTCCCTGAGAACTCCAC |
| scn3B | NM_153522.2 | β3 | CCCCTAGCTTCTCTAGTGCTCA | CTGTCTCCGAGGGTACTTCTACA |
| scn4B | NM_001013390.2 | β4 | TGAAGAAGACCCGAGAGAAGA | ACCCGTTCTCTGTGTTGTCA |
| Gusb | NM_010368.1 | Gus | CTCTGGTGGCCTTACCTGAT | CAGTTGTTGTCACCTTCACCTC |
| Hprt | NM_013556.2 | Hprt | AGGACCTCTCGAAGTGT | ATTCAAATCCCTGAAGTACTCAT |
| Gapdh | NM_008084.2 | Gapdh | CCGGGGCTGGCATTGCTCTC | GGGGTGGGTGGTCCAGGGTT |