Table 3. Validation test for the quantitative determination of the five compounds (n = 6).
| Peak No. | Compound | Linearity range (μg/mL) | Calibration curve | Correlation coefficient | LOD (ng/mL) | LOQ(ng/mL) | Recovery experiment | |
|---|---|---|---|---|---|---|---|---|
| Average recovery (%) | RSD (%) | |||||||
| 10 | Podophyllotoxin | 1–100 | Y = 0.031207X+0.038612 | 0.9994 | 2.41 | 8.04 | 102.42±2.83 | 2.78 |
| 7 | 4’-Demethylpodophyllotoxin | 1–100 | Y = 0.024322X+0.044303 | 0.9991 | 1.93 | 7.86 | 103.61±3.12 | 3.16 |
| 8 | 4’-Demethylepipodophyllotoxin | 1–100 | Y = 0.039216X+0.065137 | 0.9995 | 2.15 | 8.01 | 101.23±2.69 | 2.73 |
| 15 | Quercetin | 0.5–50 | Y = 0.012137X+0.006341 | 0.9996 | 2.92 | 9.88 | 98.25 ± 5.27 | 5.33 |
| 14 | Kaempferol | 0.5–50 | Y = 0.021675X+0.018776 | 0.9993 | 3.07 | 10.23 | 98.68 ± 5.34 | 5.38 |
Note: LOD—Litnit of Detection, LOQ—Limit of quantitation, RSD—Relative standard deviation.