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. 2015 Apr 15;10(4):e0122981. doi: 10.1371/journal.pone.0122981

Table 3. Validation test for the quantitative determination of the five compounds (n = 6).

Peak No. Compound Linearity range (μg/mL) Calibration curve Correlation coefficient LOD (ng/mL) LOQ(ng/mL) Recovery experiment
Average recovery (%) RSD (%)
10 Podophyllotoxin 1–100 Y = 0.031207X+0.038612 0.9994 2.41 8.04 102.42±2.83 2.78
7 4’-Demethylpodophyllotoxin 1–100 Y = 0.024322X+0.044303 0.9991 1.93 7.86 103.61±3.12 3.16
8 4’-Demethylepipodophyllotoxin 1–100 Y = 0.039216X+0.065137 0.9995 2.15 8.01 101.23±2.69 2.73
15 Quercetin 0.5–50 Y = 0.012137X+0.006341 0.9996 2.92 9.88 98.25 ± 5.27 5.33
14 Kaempferol 0.5–50 Y = 0.021675X+0.018776 0.9993 3.07 10.23 98.68 ± 5.34 5.38

Note: LOD—Litnit of Detection, LOQ—Limit of quantitation, RSD—Relative standard deviation.