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Vector maps for unnatural amino acid incorporation into proteins in E. coli. (A) The pEVOL vector optimized for efficient expression of orthogonal pairs of tRNA/aminoacyl-tRNA synthetase (Young et al. 2010). (B) Modified pET31b(+) vector for HQA-incorporated membrane protein expression as a KSI-fusion protein containing a thrombin cleavage site between KSI and the target membrane protein.
