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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Biomol NMR. 2014 Nov 28;61(0):185–196. doi: 10.1007/s10858-014-9884-5

Figure 3.

Figure 3

Schematic drawings of the proteins prepared by using two expression vectors shown in Figure 2 (top) and a SDS-PAGE showing the purification of a HQA-incorporated membrane protein p7 (bottom): lane 1, inclusion bodies solubilized in detergents; lane 2, flow through of the Ni-affinity chromatography; lane 3, elution from the Ni-affinity chromatography; lane 4, HPLC pure W48HQA p7 after CNBr cleavage. Note that the His-tag attached to the C-terminus of the target protein facilitates the separation of the fully transcribed proteins from the early-terminated proteins using Ni-affinity chromatography.