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Expanded region of the 15N-edited 1H spectra of uniformly 15N-labeled IL-8 in DHPC micelles. (A) IL-8 alone. (B) IL-8 bound to unlabeled W10HQA 1TM-CXCR1. (C) IL-8 bound to unlabeled W10HQA 1TM-CXCR1 in the presence of MnCl2. Close proximity between residues K20 and W52ε of IL-8 and the paramagnetic HQA-Mn2+ group at residue 10 of CXCR1 is shown by observation of select intermolecular PREs.
