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. Author manuscript; available in PMC: 2016 Mar 25.
Published in final edited form as: Cells Tissues Organs. 2015 Mar 25;199(0):311–328. doi: 10.1159/000371343

Figure 4. Effects of withdrawal of FGF2 on mineralization and the expression of markers of mineralization and dentinogenesis in primary dental pulp cultures.

Figure 4

Cultures were treated with VH or 20 ng/ml FGF2 between days 3–14. At day 14, FGF2 was withdrawn and cells were grown for additional 7 days in the medium without FGF2.

(A) Images of the same areas in cultures at day 21 analyzed under brightfield (upper row) and epifluorescent light using TRITC Red filter for detection of XO staining (middle row). The lower row shows representative von Kossa-stained dishes. The magnifications of all micrographs are identical. Scale bar = 200 µm.

(B) Histograms showing the changes in the intensity of XO staining (relative values). Levels of expression of all mRNAs were normalized to those of VH at day 14, which is arbitrarily set to 1 and indicated by the dashed line.

Results in all histograms represent mean ± SEM of values from at least three independent experiments; *p ≤ 0.05 relative to VH at each time point. Note almost complete recovery in the extent of mineralization and in the levels of expression of Dmp1 and Dspp in cultures after withdrawal of FGF2 for 7 days.