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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Biomol NMR. 2015 Jan 29;61(0):209–226. doi: 10.1007/s10858-015-9898-7

Fig. 3. 1H-13C HSQC spectra of 13C-Met labeled ClC-ec1.

Fig. 3

a Wildtype ClC-ec1 (homodimer, 100 kDa), methionine methyl region.

b Monomeric ClC-ec1 (I201W/I422W, 50 kDa).

c [H+]-dependence of 1H-13C HSQC spectra: blue, pH 7.5; orange, pH 5.0; black, reversal to pH 7.5. The asterisk (*) and dagger (†) indicate the most clearly resolved crosspeaks that exhibit H+-dependency.

d Partially deuterated (∼80%) monomeric ClCe-c1 with prolonged data collection. e Size exclusion chromatograms of ClC-ec1. Top WT ClC-ec1; Middle monomeric ClC-ec1 (mClC-ec1) (I201W/I422W); Bottom Δ6 mClC-ec1 (mutating six Met residues as follows: M38L, M65I, M228V, M276T, M330V, and M332L) yields a mix of dimer and monomer populations.