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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Pathol. 2015 Feb 19;236(1):30–40. doi: 10.1002/path.4508

Figure 6. Deletion of PTEN in podocytes accelerates the development of DN and proteinuria in mice fed a high fat diet.

Figure 6

A: iPPKO and control (CTL) mice were fed with a normal diet (ND) or a high fat diet (HFD) for 12 weeks. Body weight, blood glucose and free fatty acid were measured in each group (SEM, n=9, *p<0.01).

B: Kidney weight/tibia length ratio in each group. C: 24 h albumin excretion of control and iPPKO mice fed with ND or HFD was assessed using SDS electrophoresis (n=9, *p<0.01). D: Periodic acid–Schiff staining of glomeruli from control and iPPKO mice fed with ND or HFD. E: Quantification of mesangial matrix index in control and iPPKO mice fed with ND or HFD; 25 glomeruli/mouse were examined (n=9 in each group, *p<0.05). F: Representative electron microscopy images of glomerular basement membrane (GBM) thickening. G: quantification of GBM thickness in each group. H: Representative Western blot of isolated glomeruli lysates from control and iPPKO mice fed with ND or HFD. GAPDH was used as loading control.