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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Mar 6;1848(6):1308–1318. doi: 10.1016/j.bbamem.2015.03.001

Figure 2.

Figure 2

Size and transfection efficiency (TE) of MVL5-based CL–DNA complexes and NPs. (A) Schematic showing the internal nano-structure and functionalized surface of a PEGylated and RGD-tagged CL–DNA nanoparticle. (B) Dynamic light scattering shows that CL–DNA complexes lacking PEGylation form micron-sized aggregates when complexed in the cell culture medium DMEM. (blue curve). PEGylation with 10 mol% PEG2K-lipid or RGD-PEG2K-lipid induces the formation of sub-200 nm, sterically-stabilized nanoparticles (red and green curves). (C) MVL5/DOPC complexes show remarkably high TE, outperforming the commercial reagent Lipofectamine® 2000. As the concentration of PEG2K-lipid increases (blue bars), TE decreases. RGD-tagging of MVL5-based NPs partially recovers TE (red bars) relative to the PEGylated NPs lacking RGD. Low-σM NPs contain 30/60/10 DOTAP/DOPC/RGD-PEG2Klipid by mol% and show low TE.