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. 2015 Mar 23;5:257–263. doi: 10.1016/j.fob.2015.03.009

Fig. 2.

Fig. 2

ABCC6 deficiency in C3H and BL6-Abcc6−/− mice is associated with BMP activation in multiple organs. (A and B) Phosphorylated (p)SMAD1/5/8 and pSMAD2/3 in heart, muscle, aorta, kidneys, lungs, and liver from (A) C3H and C3H-Abcc6Tg mice and (B) BL6 and BL6-Abcc6−/− mice, as determined by immunoblotting and compared to total SMAD (n = 3 for each mouse strain). (C) PSMAD1/5/8 and pSMAD2/3 in bovine aortic endothelial cells treated with increasing concentrations of BMP4 (0–40 ng/ml) together with 10% FBS, serum from C3H or serum from C3H-Abcc6Tg mice for 24 h, as determined by immunoblotting and compared to total SMAD (n = 3 for each mouse strain).