Fig. 3.
Altered expression of ALK2 in presence of the Abcc6 transgene in C3H mice. (A and B) Expression of ALK2 in liver, lungs, kidneys, aorta, heart and muscle in C3H mice (without functional ABCC6) and in C3H-Abcc6Tg and BL6 mice (both with functional ABCC6), as determined by qPCR (expression is shown relative to that of C3H, which is set at 1) (A) and immunoblotting (B) (n = 3 for each mouse strain for qPCR and immunoblotting, respectively). Asterisks indicate statistically significant differences compared to C3H. ∗∗∗<0.001, Tukey’s test. (C) Expression of ALK2 in liver and kidneys (green), as determined by immunofluorescence. Tissues from 2 to 3 sets of mice were stained with similar results.