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. 2015 Apr 9;20(1):736–746. doi: 10.2119/molmed.2014.00146

Figure 7.

Figure 7

G-CSF induces G-CSFRIV and miR-155 expression in PBMCs from AML patients. (A) PBMCs collected from 13 AML patients and 8 healthy donors were cultured with or without 100 ng/mL G-CSF for 2 d and subjected to total RNA extraction and G-CSFRI and G-CSFRIV mRNA quantification. GAPDH levels were used as endogenous control. G-CSFRIV:G-CSFRI ratio was normalized to the respective ratio detected in the cells cultured without G-CSF. Graph displays mean ± SD of G-CSFRIV:G-CSFRI mRNA ratios. (B) PBMCs collected from 10 AML patients and 6 healthy donors were cultured with or without G-CSF for 2 d and subjected to total RNA extraction and miR-155 quantification. U6 levels were used as endogenous control. The level of miR155 was normalized to the respective level detected in the cells cultured without G-CSF. Graph displays RQ values (mean ± SD) of miR-155. Statistically significant differences were calculated using a two-tailed Student t test and are shown with asterisks (*p < 0.05 and **p < 0.01).