GAPDH |
S: 5’-CCT TCA TTG ACC TCA ACT AC -3’ |
60 °C; 30× |
593 bp |
14 |
|
A: 5’-GGA AGG CCA TGC CAG TGA GC-3’ |
Thy1 |
S: 5’-CGC TTT ATC AAG GTC CTT ACT C-3’ |
52 °C; 29× |
343 bp |
39 |
|
A: 5’-GCG TTT TGA GAT ATT TGA AGG T-3’ |
CK-18 |
S: 5’-GGA CCT CAG CAA GAT CAT GGC-3 |
60 °C; 30× |
518 bp |
|
A: 5’-CCA CGA TCT TAC GGG TAG TTG-3 |
CK-19 |
S: 5’-ACC ATG CAG AAC CTG AAC GAT-3’ |
60 °C; 30× |
261 bp |
30 |
|
A: 5’-CAC CTC CAG CTC GCC ATT AG-3’ |
AFP I. |
S I: 5’-AAC AGC AGA GTG CTG CAA AC-3 |
55 °C; 35× |
13 |
|
A I: 5’-AGG TTT CGT CCC TCA GAA AG-3’ |
AFP II. (nested) |
S II: 5’-CAC CAT CGA GCT CGC CTA TT-3 |
60 °C; 30× |
619 bp |
13 |
|
A II: 5’-TGA TGC AGA GCC TCC TGT TG-3’ |
Albumin |
S: 5’-ATA CAC CCA GAA AGC ACC TC-3’ |
60 °C; 30× |
416 bp |
13 |
|
A: 5’-CAC GAA TTG TGC GAA TGT CAC-3’ |