Table 1.
Selection of significantly regulated genes (fold change >2) upon stimulation of ephrin-B pathways in IEC-6 cells
| Gene bank accession | Title | Function | FC (30 min1) | FC (120 min1) |
| gb:AA944459 | Dynein, cytoplasmic, light intermediate chain 1 | Migration/polarity/Wound edge | -2 | NC |
| gb:AA875047 | JQ0866 T-complex protein 1 – rat | Chaperonin/protein folding | -2 | NC |
| gb:AI231350 | Transcribed sequences | Unknown | -2 | NC |
| gb:AI071071 | Transcribed sequences | Unknown | -2 | NC |
| gb:BE097926 | Transcribed sequences | Unknown | -2.1 | NC |
| gb:AA925921 | ELV4_RAT ELAV-like protein 4 | Binds to the AU-rich element in c-fos | -2.1 | NC |
| and interleukin-3/mRNA degradation | ||||
| gb:AW144020 | Transcribed sequences | Unknown | -2.1 | NC |
| gb:U30789.1 | Upregulated by 1,25-dihydroxyvitamin D-3 | Unknown | -2.3 | -2.8 |
| gb:AI230596 | Importin alpha S1 | Nuclear transport/DNA binding | -2.3 | NC |
| gb:BF389856 | Beta-galactosidase, alpha peptide | Metabolism | -2.3 | NC |
| gb:NM_012903.1 | Acidic nuc. phosphoprotein 32 family, member A | Apoptosis | -3.2 | NC |
| gb:BF415939 | c-fos | Wound healing | 16 | NC |
| gb:NM_053633.1 | Early growth response 2 | Apoptosis/EGR2 induces apoptosis | 7.5 | NC |
| gb:NM_024360.1 | HES-1/hairy and enhancer of split 1 (Drosophila) | Notch pathway target/neurite | 4.9 | NC |
| outgrowth/morphogenesis | ||||
| gb:NM_012551.1 | Early growth response 1 | Apoptosis/EGR2 induces apoptosis | 4.6 | NC |
| gb:U03389.1 | Cox-2/prostaglandin-endoperoxide synthase 2 | Epithelial wound healing | 4.6 | 4 |
| gb:BE110108 | MAP kinase phosphatase-1/protein | Signaling/stress | 3.5 | NC |
| tyrosine phosphatase | ||||
| gb:NM_021836.1 | Jun B proto-oncogene | Transcription factor/wound healing cornea | 3 | NC |
| gb:AI178746 | Transcribed sequences | Unknown | 3 | NC |
| gb:NM_012620.1 | PAI-1/serine (or cysteine) proteinase | Affect wound healing by regulating the | 2.8 | NC |
| inhibitor, member 1 | fibrinolytic environment | |||
| gb:AI710284 | Beta-galactosidase, alpha peptide | Unknown | 2.8 | 3.2 |
| gb:BF420059 | Immediate-early protein pip92 | Unknown | 2.6 | NC |
| gb:BI288619 | c-jun homolog/v-jun sarcoma virus 17 | Transcription factor/wound repair/epithelial | 2.6 | NC |
| oncogene homolog (avian) | migration | |||
| gb:BI298889 | RBBP-2 | Transcription factor/cell cycle inhibition | 2.6 | NC |
| gb:NM_031642.1 | Core promoter element binding protein | Unknown | 2.5 | NC |
| gb:AB025017 | Zinc finger protein 36 | Unknown | 2.5 | NC |
| gb:AI599423 | DNA-damage-inducible protein GADD45 gamma | Cell cycle control | 2.5 | NC |
| gb:U02553.1 | MAP kinase phosphatase-1/protein | Signaling/stress | 2.3 | NC |
| tyrosine phosphatase | ||||
| gb:L81174.1 | Ankyrin-like repeat protein | Endothelial activation | 2.1 | NC |
| gb:NM_017206.1 | Solute carrier family 6, member 6 | Protects IECs from osmotic stress | 2.1 | NC |
| gb:BM383427 | Gp130/Interleukin 6 signal transducer | Host defense/tight junction loosening/ | 2.1 | 2.3 |
| proinflammatory lipid production | ||||
| gb:AI179464 | Rattus norvegicus RM1 mRNA, partial sequence | Unknown | 2.1 | 3.2 |
| gb:NM_031530.1 | MCP-1/small inducible cytokine A2 | Monocyte chemotaxis/candidate locus for exp. EM | 2 | NC |
| gb:NM_022858.1 | HNF-3/forkhead homolog-1 | Transcription factor/development | 2 | 2.6 |
| gb:AA850780 | Transcribed sequences | Unknown | 2 | NC |
| gb:NM_012940.1 | Cytochrome P450, subfamily 1B, polypeptide 1 | Detoxification | 2 | 5.3 |
| gb:NM_013057.1 | Coagulation factor 3 | Blood coagulation | 2 | NC |
| gb:BI284349 | Myeloid differentiation primary response gene 116 | Unknown | 2 | NC |
| gb:AI176519 | DIF-2/lipid responsive gene/Immediate | Differentiation | 2 | NC |
| early response 3 protein | ||||
| gb:AI169756 | G33_rat gene 33 polypeptide | Migration/signaling/adapter protein | 2 | NC |
| binds GTP-Cdc42/activates SAPK/JNK | ||||
| gb:BI288619 | c-jun homolog/v-jun sarcoma virus | Transcription factor/wound repair/epithelial | 2 | NC |
| 17 oncogene homolog (avian) | migration | |||
| gb:AA800192 | Rattus norvegicus endogenous | Unknown | 2 | NC |
| retrovirus mRNA, partial sequence | ||||
| gb:AW533292 | Similarity to protein ref: NP_073600.1 (H. sapiens) | Unknown | 2 | NC |
| gb:BF392456 | Rattus norvegicus non-erythrocyte | Unknown | 2 | NC |
| beta-spectrin mRNA, partial cds |
1IEC-6 cells were treated with 0.5 mg/mL (- 3.3 nmol/L) rat recombinant EphB1-Fc and RNA was harvested after 30 and 120 min. FC: fold changes; NC: no changes.