Table 1.
Primer sequences and restriction enzymes of mutation in Se and Le genes
| Mutation | Primer sequence | Enzyme |
| Se gene | ||
| A385T | UP1: GATGGAGGAGGAATACCGCCTC | Ear I |
| DP2: GATCTCCTGGCGGAGGTGGTGGTAGAAGATC | ||
| G428A | Identical to A358T primer pairs | Bgl II |
| C571T | UP: AGGAGATCCTCCAGGAGTTCA | Dde I |
| DP: AGAAGGAGAAAAGGTCTCAAAGG | ||
| G849A | Identical to C571T primer pairs | Dde I |
| C628T | UP: AGTGTGGAAGGGGGTGGTGCCC | Bgl I |
| DP: CCACTCTGGCAGGAAGGC | ||
| Fusion gene | UP: CTGCCTCCTGACCATGTCC | Pst I |
| DP: identical to reverse primer of C628T | ||
| Le genes | ||
| T202C and C314T | UP: CCACCCTCCTGATCCTGCTC | Msp I (T202C) |
| DP: GATATCCCAGTGGTGCACGATGATGATC | Bcl I (C314T) | |
| C445A | UP: identical to UP of T202 C and C314 T | BstN I |
| DP: GAGATTGAAGTATCTGTCCAAGGC | ||
| G508A | UP: TCAACTTGGAGCCACACCCT | Alu I |
| DP: AGTTGGACACCGCCCAGGCCACCAG | ||
| A1007C | UP: GCTCCTTCCGCTGGGCACTAG | Alu I |
| DP: TGGCCACAAAGGACTCCAGC | ||
| T1067A | UP: GTACCAGACGGTGCGATGCA | Nsi I |
| DP: identical to DP of A1007 C |
1UP: upstream primer; 2DP: downstream primer; The mutated base is underlined.