Table 1.

Primers Used to Obtain the rpS6 WT Clone or Quadruple Phosphomimetic Mutant

Target Gene	GenBank Accession Number	Primer Sequence (5′-3′)	Nucleotide Position	Size in b.p.
Primers
rpS6	NM_017160.1	S, 5′-AAACGCGTATGAAGCTGAATATCTCCTTC-3′	1–21	766
		AS, 5′-AATCTAGATTATTTTTGACTGGACTCAGA-3′	730–750
Mutagenic primers
rpS6	NM_017160.1	S, 5′-AAACGCGTATGAAGCTGAATATCTCCTTC-3′	1–21	766
AS, 5′-AATCTAGATTATTTTTGACTGGACTCTTCTTTAGAAGTTTCAGCTCTCAGTTCTTC-3′			703–750

Underlined italicized sequences and underlined boldface/italicized sequences indicate MluI and XbaI restriction enzyme recognition site, respectively, which were added for cloning of the WT rpS6 or constitutively active quadruple phosphomimetic rpS6 mutant into the pCI-neo mammalian expression vector. Underlined sequences indicate mutation sites in which Ser residues were replaced by Glu to obtain the quadruple phosphomimetic rpS6 mutant. S, sense; AS, antisense.