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. 2015 Feb 23;156(5):1630–1636. doi: 10.1210/en.2014-1980

Figure 1.

Figure 1.

LC-MS qTOF monitoring of melatonin transformation to AMK by human epidermal keratinocytes and melanoma cells. A, Although production of AMK is undetectable in HaCaT keratinocytes incubated without substrate (inset), AMK is easily detectable (m/z = 259.1 [M+Na]+) in cells cultured in the presence of melatonin. B, Dose-dependent production of AMK by HaCaT keratinocytes. C and D, Production of AMK (m/z = 237.1 [M+H]+) in SKMEL-188 cells is enhanced in pigmented (C) in comparison with nonpigmented cells (D). The insets show controls (cells incubated without substrate) and color of cell pellets corresponding to the melanin content. Arrows show RT of AMK standard. Melatonin was added at concentration 500μM in C and D.