Detection of AMK in the human epidermis. A, Detection of an AMK ion at m/z = 259.1 [M+Na]+ with RT of the AMK standard. B–F, Levels of endogenous production of AMK in relation to race, age, and gender. B, All subjects compared. C and D, AAs. E and F, Cs. Quantification was performed using ion at m/z = 237.1 [M+H]+, and data were calculated from the standard curve obtained with chemically synthesized AMK. **, P < .01 at Student's t test.