Fig. 2.

Hyperglycemia and acute glycemic fluctuation enhances MG occludin glycation. IHECs were grown under normal glucose (5 mM) or high glucose (25 mM glucose for 7 days or 12 days) or in cells subjected to acute glycemic fluctuation (25 mM glucose for 12 days, then change from 25→5 mM glucose for 6 h). Total occludin and MG–occludin glycated adducts were determined in whole cell lysates by Western blot analyses. Representative blots were shown. The right panels show the quantification of occludin or MG–occludin band intensities relative to β-actin (mean±SEM) for 6 separate immunoblots. (A) Occludin content and (B) contents of MG–occludin adducts without or after 300 µM MG treatment for 8 h. The bars with similar alphabets are significantly different from one another, p<0.05.