Fig. 3.

N-acetylcysteine (NAC), a GSH precursor, attenuates MG-induced IHEC dysfunction under conditions of hyperglycemia and acute glycemic fluctuation. TEER loss was induced by 300 or 600 µM MG in IHECs adapted to high glucose (12 days 25 mM, A) or high glucose and then acutely transferred to 5 mM glucose (25→5 mM, B). TEER measurements were performed in the absence or presence of 2 mM NAC as described in Section Methods. ^⁎p<0.05 vs MG without NAC, n=3. (C) Effect of NAC on cellular GSH levels in high glucose-adapted IHECs (25 mM) or in cells subjected to acute glycemic fluctuation (25→5 mM). ^⁎p<0.05 vs untreated cells; ^#p<0.05 vs MG alone, n=3.