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. 2015 Feb 28;5:5–9. doi: 10.1016/j.nmni.2015.02.002

Table 1.

Details of the primers, amplification temperature and amplicon size used in the study

Sl No. Gene Oligonucleotide sequence (5′–3′) Amplification temp (°C) Amplicon size (bp)
1 NDM-1 ACCGCCTGGACCGATGACCA 58°C 264
GCCAAAGTTGGGCGCGGTTG
2 gyrA GACGGCCTGAAGCCGGTGCAC 64°C 417
GCCCACGGCGATACCGCTGGA
3 gyrB AGTACTTCGCCGACTTCCT 739
TACAGGCGCGACAGGCGCTT
4 parC TCTACGCCATGAGCGAACTGG 262
AGCAGCACCTCGGAA TAGCG
6 qnrA ATTTCTCACGCCAGGATTTG 64°C 516
GATCGGCAAAGGTTAGGTCA
7 qnrB GATCGTGAAAGCCAGAAAGG 476
ATGAGCAACGATGCCTGGTA
8 qnrC GGGTTGTACATTTATTGAATCG 307
CACCTACCCATTTATTTTCA
9 qnrS GCAAGTTCATTGAACAGGGT 428
TCTAAACCGTCGAGTTCGGCG
10 aac (6′)-Ib-cr TTGCGATGCTCTATGAGTGGCTA 55°C 482
CTCGAATGCCTGGCGTGTTT
11 qepA AACTGCTTGAGCCCGTAGAT 596
GTCTACGCCATGGACCTCAC
12 blaTEM GAGTATTCAACATTTTCGT 50°C 857
ACCAATGCTTAATCAGTGA
13 blaSHV TCGCCTGTGTATTATCTCCC 768
CGCAGATAAATCACCACAATG
14 blaCTX-M-3 AATCACTGCGTCAGTTCAC 701
TTTATCCCCCACAACCCAG
15 blaOXA1 GCAGCGCCAGTGCATCAAC 198
CCGCATCAAATGCCATAAGTG
16 blaOXA7 AGTTCTCTGCCGAAGCC 591
TCTCAACCCAACCAACCC