Table 1.
Sl No. | Gene | Oligonucleotide sequence (5′–3′) | Amplification temp (°C) | Amplicon size (bp) |
---|---|---|---|---|
1 | NDM-1 | ACCGCCTGGACCGATGACCA | 58°C | 264 |
GCCAAAGTTGGGCGCGGTTG | ||||
2 | gyrA | GACGGCCTGAAGCCGGTGCAC | 64°C | 417 |
GCCCACGGCGATACCGCTGGA | ||||
3 | gyrB | AGTACTTCGCCGACTTCCT | 739 | |
TACAGGCGCGACAGGCGCTT | ||||
4 | parC | TCTACGCCATGAGCGAACTGG | 262 | |
AGCAGCACCTCGGAA TAGCG | ||||
6 | qnrA | ATTTCTCACGCCAGGATTTG | 64°C | 516 |
GATCGGCAAAGGTTAGGTCA | ||||
7 | qnrB | GATCGTGAAAGCCAGAAAGG | 476 | |
ATGAGCAACGATGCCTGGTA | ||||
8 | qnrC | GGGTTGTACATTTATTGAATCG | 307 | |
CACCTACCCATTTATTTTCA | ||||
9 | qnrS | GCAAGTTCATTGAACAGGGT | 428 | |
TCTAAACCGTCGAGTTCGGCG | ||||
10 | aac (6′)-Ib-cr | TTGCGATGCTCTATGAGTGGCTA | 55°C | 482 |
CTCGAATGCCTGGCGTGTTT | ||||
11 | qepA | AACTGCTTGAGCCCGTAGAT | 596 | |
GTCTACGCCATGGACCTCAC | ||||
12 | blaTEM | GAGTATTCAACATTTTCGT | 50°C | 857 |
ACCAATGCTTAATCAGTGA | ||||
13 | blaSHV | TCGCCTGTGTATTATCTCCC | 768 | |
CGCAGATAAATCACCACAATG | ||||
14 | blaCTX-M-3 | AATCACTGCGTCAGTTCAC | 701 | |
TTTATCCCCCACAACCCAG | ||||
15 | blaOXA1 | GCAGCGCCAGTGCATCAAC | 198 | |
CCGCATCAAATGCCATAAGTG | ||||
16 | blaOXA7 | AGTTCTCTGCCGAAGCC | 591 | |
TCTCAACCCAACCAACCC |