Table 5.
5 nl/min |
20 nl/min |
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Group | n | JVa , pmol·min−1·mm−1 | n | JVb, pmol·min−1·mm−1 | ΔJV (JVb − JVa) | ΔJV/JVa*100 | (ΔJV/JVa)/(ΔT/Tr) |
Control (1.2 mM CaCl2) | 15 | 0.84 ± 0.03 | 15 | 1.36 ± 0.04*** | 0.52 ± 0.05 | 62.31 ± 4.5 | 1.42 ± 0.10 |
5 mM CaCl2 | 6 | 0.55 ± 0.09††† | 6 | 0.78 ± 0.11ns††† | 0.23 ± 0.14† | 42.04 ± 19.7NS | 0.64 ± 0.30†† |
0 mM CaCl2+EGTA | 6 | 0.81 ± 0.06NS | 6 | 1.37 ± 0.08***NS | 0.55 ± 0.10NS | 67.75 ± 9.9NS | 1.83 ± 0.27NS |
BAPTA+0 mM CaCl2+ EGTA | 6 | 0.93 ± 0.12NS | 6 | 0.77 ± 0.06ns††† | −0.15 ± 0.13††† | −16.72 ± 6.0††† | −0.42 ± 0.15††† |
Thapsigargin | 6 | 0.50 ± 0.06††† | 6 | 0.74 ± 0.07*††† | 0.23 ± 0.09†† | −46.61 ± 13.5NS | 1.17 ± 0.34NS |
2-APB | 7 | 0.79 ± 0.05NS | 7 | 0.61 ± 0.08ns††† | −0.17 ± 0.09††† | −22.16 ± 10.7††† | −0.41 ± 0.20††† |
Values are means ± SE; n, no. of perfused tubules; Jv, fluid absorption; ΔJv, the differences in Jv between perfusion rate of 5 and 20 nl/min; ΔJV/JVa*100, percent changes in fluid reabsorption from low flow rate. ns, Not significantly different from low flow rates in the same group;
Significant difference from low flow rates in the same group (*P < 0.05, **P < 0.01, ***P < 0.001). NS, not significantly different compared with control at the similar flow rates.
Significant difference compared with control at the similar flow rates (†P < 0.05, ††P < 0.01, †††P < 0.001).