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. 2015 Jan 28;308(8):F839–F847. doi: 10.1152/ajprenal.00601.2014

Table 5.

Effects of low and high luminal calcium concentration, calcium chelator, Ca-ATPase inhibitor, and IP3 receptor antagonist on flow-induced changes in fluid absorption under low and high perfusion rates in mouse proximal tubules

5 nl/min
20 nl/min
Group n JVa , pmol·min−1·mm−1 n JVb, pmol·min−1·mm−1 ΔJV (JVbJVa) ΔJV/JVa*100 JV/JVa)/(ΔT/Tr)
Control (1.2 mM CaCl2) 15 0.84 ± 0.03 15 1.36 ± 0.04*** 0.52 ± 0.05 62.31 ± 4.5 1.42 ± 0.10
5 mM CaCl2 6 0.55 ± 0.09††† 6 0.78 ± 0.11ns††† 0.23 ± 0.14 42.04 ± 19.7NS 0.64 ± 0.30††
0 mM CaCl2+EGTA 6 0.81 ± 0.06NS 6 1.37 ± 0.08***NS 0.55 ± 0.10NS 67.75 ± 9.9NS 1.83 ± 0.27NS
BAPTA+0 mM CaCl2+ EGTA 6 0.93 ± 0.12NS 6 0.77 ± 0.06ns††† −0.15 ± 0.13††† −16.72 ± 6.0††† −0.42 ± 0.15†††
Thapsigargin 6 0.50 ± 0.06††† 6 0.74 ± 0.07*††† 0.23 ± 0.09†† −46.61 ± 13.5NS 1.17 ± 0.34NS
2-APB 7 0.79 ± 0.05NS 7 0.61 ± 0.08ns††† −0.17 ± 0.09††† −22.16 ± 10.7††† −0.41 ± 0.20†††

Values are means ± SE; n, no. of perfused tubules; Jv, fluid absorption; ΔJv, the differences in Jv between perfusion rate of 5 and 20 nl/min; ΔJV/JVa*100, percent changes in fluid reabsorption from low flow rate. ns, Not significantly different from low flow rates in the same group;

*

Significant difference from low flow rates in the same group (*P < 0.05, **P < 0.01, ***P < 0.001). NS, not significantly different compared with control at the similar flow rates.

Significant difference compared with control at the similar flow rates (†P < 0.05, ††P < 0.01, †††P < 0.001).