Fig. 8.

P30 Fgfr2^BM−/− bladders have reduced muscle and increased collagen. A and A′: low-power H&E-stained images of P30 control and Fgfr2^BM−/− bladders showing comparable overall sizes but decreased mutant bladder wall thickness versus controls. B and B′: higher-power analysis indicating a thinner and less compact muscle layer in P30 Fgfr2^BM−/− (A′, arrowhead) compared with age-matched control (A) bladders. *Lumen. C and C′: trichrome staining showing increased collagen deposition (white arrows) in the detrusor layer of P1 Fgfr2^BM−/− (B′) compared with age-matched control (B) bladders. D: quantitative PCR showing significantly reduced α-SMA mRNA expression in P30 Fgfr2^BM−/− bladders than in control bladders. E: Western blots demonstrating reduced α-SMA protein in P30 Fgfr2^BM−/− compared with age-matched control bladders. α/β- Tubulin was used as a loading control. F and G: quantitative PCR showing increased Col1a and Col3a mRNA expression in P30 Fgfr2^BM−/− versus age-matched control bladders. H: graph showing increased collagen in whole P30 Fgfr2^BM−/− bladder lysates compared with age-matched control bladder lysates. Scale bar = 700μm in A and A′, 500 μm in B and B′, and 300 μm in C and C′.Values are means ± SD. *P < 0.05; ***P < 0.001.