Fig. 7.

Blockade of CXCR4 mobilizes CD31⁺/CD45⁺ from the bone marrow to the blood after liver I/R. Mice were subjected to hepatic I/R injury or sham procedure and injected intraperitoneally with PBS (vehicle), AMD3100 (10 mg/kg), or recombinant SDF-1 (1 μg) every 12 h starting 24 h after I/R injury. CD31⁺/CD45⁺ cells were assessed by flow cytometry in bone marrow (A) and peripheral blood (B). Treatment with AMD3100 resulted in a decrease in the number of CD31⁺/CD45⁺ cells in the bone marrow and an increase in the peripheral circulation. Values are means ± SE; n = 4 per group. *P < 0.05 vs. control. **P < 0.05 vs. all groups. C: engraftment of CD45 cells in liver. Liver sections from C57BL/6 mice were obtained 1 h after adoptive transfer of 1.5 × 10⁵ mononuclear cells isolated after I/R injury from C57BL/6 mice, which express CD45.1 on hematopoietic cells. Number of CD45.1 cells per high-powered field (HPF) were counted. Values are means ± SE; n = 5–6 per group. *P < 0.05 vs. control.