Fig. 1.
Expression of renin-angiotension system (RAS) components [angiotensinogen (Ao), renin, angiotensin-converting enzyme (ACE), angiotensin receptor types 1a (AT1a), 1b (AT1b), and 2 (AT2)] in liver sections and cholangiocytes isolated from normal and bile duct-ligated (BDL) rats and normal rat intrahepatic cholangiocyte lines (NRIC). RAS components were assessed by fluorescence-activated cell sorting (FACS) analysis (A) and real-time PCR (B) in cholangiocytes from normal and BDL rats. *P < 0.05 vs. normal. Data are presented as means ± SE, n = 6. Expression of RAS components in NRIC cells was evaluated by immunofluorescence (C). Expression of RAS by immunohistochemistry in normal and BDL rat liver sections (D). Original magnification ×40. Black arrows indicate positively stained bile ducts.