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. 2015 Feb 20;308(8):L776–L787. doi: 10.1152/ajplung.00334.2014

Fig. 4.

Fig. 4.

17-AAG increases and LPS reduces the abundance of Hsp90/p53 complexes in HLMVEC. Top: Western blot analysis of p53 in HLMVEC treated with either vehicle (10% DMSO) or LPS for 2 h or 17-AAG for 8 h. Signal intensity of p53 was analyzed by densitometry. Protein levels were normalized to β-actin. *P < 0.05, **P < 0.01, vs. vehicle. Means ± SE. Bottom: Western blot analysis of samples at top, after immunoprecipitation (IP) with antibody against p53 and immunoblotting (IB) for p53 and Hsp90. Each blot is representative of 3 independent experiments. *P < 0.05, ****P < 0.0001, vs. vehicle. Means ± SE.