Fig. 7.
17-AAG suppresses the LPS-induced p53 phosphorylation. A: Western blot analysis of p-p53Ser15, p-p53Ser392, and p53 expression levels in HLMVEC treated with LPS or vehicle and pretreated with 17-AAG or vehicle (10% DMSO). Blot shown is representative of 4 independent experiments. Signal intensity of p-p53Ser15 (B and C) and p-p53392 (D and E) was analyzed by densitometry. Protein levels were normalized to p53 (B and D) or β (C and E) actin. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. corresponding vehicle-treated cells. NS, nonsignificant. Means ± SE.