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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Nature. 2015 Mar 25;520(7546):248–252. doi: 10.1038/nature14337

Extended Data Figure 10. Formation of ectopic heterochromatin.

Extended Data Figure 10

a, Differential gene expression compared to differential antisense siRNA expression in leo1Δ. Gene expression profiles were obtained with whole-genome tiling arrays and small RNA profiles by deep sequencing. Genes neighboring the nmt1+::ade6-hp+, trp1+::ade6+, and ade6-704 loci are marked in color (see also Supplementary Table 1). b, siRNA reads mapping to the ade6-704 locus in leo1+ and leo1Δ strains. Red, plus strand; blue, minus strand. Intronic rpl2302 siRNAs in leo1Δ cells indicate co-transcriptional dsRNA synthesis by RDRC prior to splicing. c, ChIP experiment showing H3K9me2 enrichments on genes surrounding the ade6-704 locus in leo1+ and leo1Δ cells. Enrichments were calculated relative to background levels obtained in clr4Δ cells and normalized to adh1+. Error bars, SD; mean of n=2 independent biological replicates.