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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Nature. 2015 Mar 25;520(7546):248–252. doi: 10.1038/nature14337

Extended Data Figure 6. ade6+ siRNAs trigger de novo methylation of H3K9 at homologous ade6+ sequences in cis and in trans.

Extended Data Figure 6

a, ade6-hp RNA producing locus and siRNA targe loci in trans in the sms0 strain. ade6-704 is a loss-function allele of the endogenous ade6+ gene and serves as a positive control in the silencing assays. b and c, ade6+ siRNAs direct the methylation of H3K9 at ade6 targets in cis (green) and in trans (red) in Paf1C mutant cells. H3K9me2 for tpr1+::ade6+ is shown in figure 1d. Quantitative PCR was performed with locus-specific primers. Error bars, SEM; n=3 technical replicates. d and e, ChIP experiments to assess ade6+ transcriptional activity. H3K36me3 levels were normalized to total H3 levels. snu6+ is transcribed by RNAPIII and serves as background control. Error bars, SEM; n=3 independent biological replicates; p values were calculated using the one-tailed Student’s t test.