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TNFα and Il1β mRNA expression in cholesteatoma. TNFα and Il1β mRNA expression in cholesteatoma compared to external auditory canal skin (EAS). Real time PCR reveals a significantly higher expression of TNFα and Il1β within the cholesteatoma compared to EAS. For normalization, the housekeeping gene GAPDH was used and compared to EAS. N = 10 samples; statistics was performed by GraphPad Prism with the use of an unpaired t-test, P < 0.05.
