Skip to main content
. 2015 Apr 9;161(2):228–239. doi: 10.1016/j.cell.2015.03.026

Figure 3.

Figure 3

PCR Validation of Somatic L1 Insertions

(A–F) Validated examples from hippocampal neuron single-cell RC-seq data included: (A) a full-length L1 insertion in neuron CTRL-42-HN-#13; (B) a truncated L1 insertion in neuron CTRL-42-HN-#11; (C) a heavily truncated L1 insertion in neuron CTRL-55-HN-#15; and (D) a very heavily truncated L1 insertion yielding a 3′ transduction in neuron CTRL-42-HN-#4, also validated in neuron CTRL-42-HN-#3, and traced to a donor L1-Ta on chromosome 3; (E) a very heavily truncated L1 insertion detected in CTRL-42-HN-#13 and validated in 10/21 CTRL-42 hippocampal neurons tested. Asterisks denote neurons where validation succeeded; (F) a very heavily truncated L1 insertion detected in CTRL-42-HN-#4 and also validated in CTRL-42-HN-#22. Note: in (A–F) the 3′ L1-genome junction was detected by single-cell RC-seq, while the 5′ L1-genome junction was identified by insertion-site PCR (using primers indicated by α and β) and sequencing. Green triangles indicate TSDs. Numbers below the 5′ L1-genome junction indicate the equivalent L1-Ta consensus position. See also Table S2 and Data S1.