Figure S6.

Signatures of L1 Mobilization via TPRT Detected by Hippocampus and Liver Bulk RC-Seq, Related to Figure 6

(A) TSD size distribution for polymorphic L1 insertions detected at their 5′ and 3′ L1-genome junctions.

(B) As for (A), except for hippocampal somatic L1 insertions.

(C) TSD size distribution for hippocampal somatic L1 insertions detected at only a 5′ L1-genome junction.

(D) Consensus L1 EN motif for liver somatic L1 insertions detected at only a 3′ L1-genome junction by RC-seq.

Expected values for (A) and (B) were calculated by randomizing sense and antisense RC-seq read cluster genomic coordinates, to ascertain how many overlapping clusters in the opposing orientation and detecting opposite ends of an L1 insertion were found, using the same bioinformatics process as used for observed clusters. Expected values for (C) were calculated by random sampling of genomic coordinates and searching for the nearest upstream L1 EN motif, again following the same string matching process as for observed values. Note: the corresponding TSD size distribution for liver somatic L1 insertions detected at only their 5′ L1-genome junction contained insufficient data (n = 7) to make a meaningful comparison with hippocampal somatic L1 insertions.