Figure 2.

During Contact Inhibition, the Actin Network Is Rapidly Reorganized in Colliding Partners

(A) Top panels are still images from a time-lapse movie of hemocytes containing labeled F-actin during a collision. While cells are in contact, an actin fiber develops between the cell body and the point of contact in colliding partners (arrowheads), which often deforms and breaks upon lamellar retraction (red arrow). Bottom panels highlight actin flow dynamics obtained from the pseudo-speckle analysis. Note that the decreased actin flow in the vicinity of lamellae overlap (highlighted by yellow arrows) is due to the inability of the algorithm to distinguish between the two networks.

(B) Kymograph of the region surrounding the actin fiber highlighting the actin retrograde flow dynamics and the alignment of the microtubule bundles (pseudocolored white).

(C and D) Instantaneous changes in retrograde flow rate quantified from lamellae contact (C) or lamellae separation (D).

(E and F) Instantaneous changes in retrograde flow direction quantified from lamellae contact (E) or lamellae separation (F).

For (C)–(F), error bars represent SD. See also Figure S2 and Movie S2.