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. 2015 Apr 9;161(2):361–373. doi: 10.1016/j.cell.2015.02.015

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S4 — Myosin II Drives Retrograde Flow in Hemocytes, Related to Figure 6

(A) Still images of hemocyte dispersal along the ventral surface of Stage 15 embryos. Hemocyte dispersal was disrupted in myosin II mutant (zip¹) embryos and expression of GFP-tagged Myosin II specifically in myosin II mutants rescued dispersal.

(B) The mean retrograde flow across the lamella of freely moving cells was calculated for control, myosin II mutant (zip¹), and myosin II mutant rescue hemocytes. ^∗p < 0.05. (C) Probability density function (PDF) of retrograde flow rates in freely moving control, myosin II mutant (zip¹), and myosin II mutant rescue hemocytes revealed that expression of GFP-tagged Myosin II increased the distribution of higher flow values in the zip¹ mutant cells.