Figure S5.
Migration of diaphanous Mutant Hemocytes, Related to Figure 6
(A) Left panel is a still image of a freely moving hemocyte containing labeled F-actin. Right panel is a heatmap of the actin retrograde flow field.
(B) The mean retrograde flow across the lamella of freely moving cells was calculated for control, and diaphanous mutant (dia5) hemocytes.
(C) Probability density function (PDF) of retrograde flow rates in freely moving control and diaphanous mutant hemocytes revealed a similar distribution.
(D) Quantification of cell speed in freely moving control and diaphanous mutant hemocytes. Error bars represent SEM.
(E) Quantification of the directional ratio (persistence) of freely moving control and diaphanous mutant hemocytes. Error bars represent SEM.
(F) Quantification of instantaneous changes in flow rate in control and diaphanous mutant hemocytes during contact inhibition. Note that the control analysis was taken from Figure 2C. Error bars represent SD.
(G) Cross correlation of the instantaneous changes in flow rate in lamellae of colliding diaphanous mutant cells. Error bars represent SEM. Red dotted lines represent the mean correlation between colliding cells immediately prior to cell-cell contact with the thickness representing the SEM. Note that there is no increase in the correlation coefficient upon lamellae contact as observed in control cells (Figure 3G).
(H) Quantification of instantaneous changes in flow direction in control and diaphanous mutant hemocytes during contact inhibition. Note that the control analysis was taken from Figure 2E. Error bars represent SD.
(I) Cross correlation of the instantaneous changes in flow direction in lamellae of colliding diaphanous mutant cells. Error bars represent SEM. Red dotted lines represent the mean correlation between colliding cells immediately prior to cell-cell contact with the thickness representing the SEM. Note that there is no increase in the correlation coefficient upon lamellae contact as observed in control cells (Figure 3I).