Fig 1.
Basal expression levels of insulin-like growth factor 1 receptor (IGF1R) protein and the correlation with intrinsic resistance to ZSTK474 in JFCR39 cell lines. (a) Cells without ZSTK474 treatment were harvested and the expression of IGF1R protein in each of the JFCR39 cell lines was examined by immunoblot analysis. Expression level of IGF1R in each sample was normalized to that in the “control” sample (mixture of lysates prepared from all 39 cells). Quantitative data are median values of three independent experiments; images show representative results. (b) Correlation between GI50 concentrations of ZSTK474 and IGF1R protein expression across the JFCR39 cancer cell lines. Pearson correlation coefficients and P-values were calculated for demonstrating statistical significance.