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. 2015 Jan 16;106(2):171–178. doi: 10.1111/cas.12582

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig 3 — Effect of insulin-like growth factor 1 receptor (IGF1R) siRNAs on activity of ZSTK474 to inhibit cell proliferation and phosphatidylinositol 3-kinase downstream signaling. (a) Knockdown of IGF1R protein expression using IGF1R siRNAs. MKN28, St-4, SNB75, and PC3 cells were transfected with control siRNA (si-cont.) or IGF1R siRNAs (siIGF1R-1 or siIGF1R-2) and the expression of IGF1R protein was measured by immunoblot analysis. (b) Dose–response curves of ZSTK474 against cell growth in MKN28, St-4, SNB75, and PC3 cells after transfection with control siRNA or IGF1R siRNAs. Cell growth was assessed by sulforhodamine B assay. Assays were carried out in duplicate and the data are representative of two independent experiments. (c) MKN28, St-4, SNB75, and PC3 cells were transfected with either control siRNA or IGF1R siRNA, and then exposed to ZSTK474 at the indicated concentrations for 3 h. Cells were then harvested and immunoblot analyses of indicated proteins were carried out.