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Effect of nitric oxide donor S-nitrosated human serum albumin (SNO-HSA) on autophagy. Colon cancer C26 cells were incubated with different concentrations (μM) of SNO-HSA for 24 h. The cells were then treated with (1st Ab (+)) or without (1st Ab (−)) an antimicrotubule-associated protein 1 light chain 3 (LC3) antibody. Fluorescence was quantified using the Guava easyCyte Flow Cytometry system. Results are given as means ± SD. *P < 0.05, **P < 0.01 versus 1st Ab alone (n = 3).
