Fig 5.

Inhibition profiles of FK-A11 for p110α isoforms. Enzyme activities of p110 isoforms, α, β, γ and δ, were measured using a homogenous time-resolved fluorescence (HTRF) assay. 10 nM wortmannin (a) or 3 μM FK-A11 (b) was incubated with a solution containing each PI3K isoform protein, 10 μM PIP2, 25 μM ATP and 2 mM DTT. Percent inhibition of the enzyme activity by the compounds was calculated with the following formula. Percent inhibition = [(readout value of inhibited activity – that of normal activity)/(readout value of biotinylated-PIP3 – that of normal activity)] × 100.