TABLE 1.
Bacterial strains and plasmids used in this studya
| Strain or plasmid | Genotype and/or relevant characteristics | Reference or Source |
|---|---|---|
| Y. pestis CO92 strains | ||
| WT CO92 | Virulent Y. pestis biovar Orientalis strain isolated in 1992 from a fatal human pneumonic plague case and naturally resistant to polymyxin B | CDC |
| WT CO92(pBR322) | WT Y. pestis CO92 transformed with pBR322 (Tcs) | 18 |
| WT CO92 luc2 | WT Y. pestis CO92 integrated with the luciferase gene (luc), used as a reporter strain | 39 |
| miniTn5Km2STM mutants | Random transposon insertion mutants of Y. pestis CO92 | This study |
| WT CO92(pKD46) | WT Y. pestis CO92 transformed with plasmid encoding phage λ recombination system | This study |
| Δypo0498 strain | ypo0498 deletion mutant of Y. pestis CO92 | This study |
| ΔrbsA strain | rbsA deletion mutant of Y. pestis CO92 | This study |
| ΔrbsA(pBR322) strain | ΔrbsA strain transformed with pBR322 (Tcs) | This study |
| ΔrbsA(pBR322-rbsA) strain | ΔrbsA strain complemented with pBR322-rbsA (Tcs) | This study |
| ΔvasK strain | vasK deletion mutant of Y. pestis CO92 | This study |
| ΔvasK(pBR322) strain | ΔvasK strain transformed with pBR322 (Tcs) | This study |
| ΔvasK(pBR322-vasK) strain | ΔvasK strain complemented with pBR322-vasK (Tcs) | This study |
| Δlpp strain | lpp deletion mutant of Y. pestis CO92 | 20 |
| Δlpp(pKD46) strain | Δlpp strain transformed with plasmid encoding phage λ recombination system | This study |
| Δlpp(pBR322) strain | Δlpp strain transformed with pBR322 (Tcs) | This study |
| Δlpp ΔrbsA strain | lpp rbsA double deletion mutant of Y. pestis CO92 | This study |
| Δlpp ΔrbsA(pBR322) strain | Δlpp ΔrbsA double mutant transformed with pBR322 (Tcs) | This study |
| Δlpp ΔrbsA(pBR322-rbsA) strain | Δlpp ΔrbsA double mutant complemented with pBR322-rbsA (Tcs) | This study |
| Δlpp ΔmsbB strain | lpp msbB double deletion mutant of Y. pestis CO92 | 19 |
| Δlpp ΔmsbB(pKD46) strain | Δlpp ΔrbsA double mutant transformed with plasmid encoding phage λ recombination system | This study |
| Δlpp ΔmsbB ΔrbsA strain | lpp msbB rbsA triple deletion mutant of Y. pestis CO92 | This study |
| Δlpp ΔmsbB ΔrbsA(pBR322) strain | Δlpp ΔmsbB ΔrbsA trible mutant transformed with pBR322 (Tcs) | This study |
| Δlpp ΔvasK strain | lpp vasK double deletion mutant of Y. pestis CO92 | This study |
| Δlpp ΔvasK(pBR322) strain | Δlpp ΔvasK double mutant transformed with pBR322 (Tcs) | This study |
| A. hydrophila strainsb | ||
| SSU | Aeromonas hydrophila human diarrheal isolate | 43 |
| ΔvasK strain | vasK deletion mutant of A. hydrophila SSU | 43 |
| E. coli S17-1(pUTminiTn5Km2STM) | E. coli S17-1, with chromosomally integrated recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 λpir, carrying a pUTminiTn5Km2STM plasmid | 35 |
| Plasmids | ||
| pUTminiTn5Km2STM | Minitransposon plasmid carrying 1 of 53 unique STM tags | 35 |
| pKD46 | Plasmid for phage λ recombination system under arabinose-inducible promoter | 37 |
| pKD13 | Template plasmid for PCR amplification of the Kmr gene cassette flanked by FRT sites | 37 |
| pFlp2 | Plasmid for FLP enzyme under constitutively expressed lac promoter (Apr) | 38 |
| pBR322 | A variant of pBR322 (Tcs) | 70 |
| pBR322-rbsA | Plasmid containing the rbsA coding region and its putative promoter inserted in the Tcr cassette of vector pBR322 | This study |
| pBR322-vasK | Plasmid containing the vasK coding region and its putative promoter inserted in the Tcr cassette of vector pBR322 | This study |
a
CDC, Centers for Disease Control and Prevention; Tcr, tetracycline resistance; Tcs, tetracycline susceptibility; Apr, ampicillin resistance; FLP, flippase; FRT, flippase recognition target.
b
A. hydrophila SSU has now been reclassified as A. dhakensis SSU (40).