TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| C. rodentium | ||
| DBS100 | Wild-type C. rodentium DBS100 | 48 |
| ΔcpxRA | DBS100 ΔcpxRA | This study |
| ΔcpxRA::cpxRA | DBS100 ΔcpxRA with a single copy of cpxRA integrated at the att Tn7 site; Gentr | This study |
| ΔcpxRA(pSTNSK) | DBS100 ΔcpxRA + pSTNSK; Kanr | This study |
| spy::lacZ | DBS100 with a chromosomal insertion of spy::lacZ | This study |
| ΔcpxRA::spy::lacZ | ΔcpxRA strain with a chromosomal insertion of spy::lacZ | This study |
| ΔcpxRA::cpxRA::spy::lacZ | ΔcpxRA::cpxRA strain with a chromosomal insertion of spy::lacZ | This study |
| E. coli | ||
| DH5α | HuA2 Δ(lac)U169 phoA glnV44 ϕ80lacZΔM15 endA recA hsdR17 (rM− mK+) thi-1 gyrA96 relA1 | Invitrogen |
| DH5α(pBSΔcpxRA) | DH5α containing pBSΔcpxRA | This study |
| DH5αλpir | K-12 F− ϕ80lacZΔM15 endA recA hsdR17 (rM− mK+) supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 λpir | |
| DH5αλpir(pΔcpxRA) | DH5αλpir containing pΔcpxRA | This study |
| DH5αλpir(pcpxRA) | DH5αλpir containing pcpxRA | This study |
| DH5αλpir(pspylacZ) | DH5αλpir containing pspylacZ | This study |
| χ7213 | thr-1 leuB6 fhuA21 lacY1 glnV44 recA1 asdA4 thi-1 RP4-2-Tc::Mu [-pir] Kanr | 49 |
| χ7213(pΔcpxRA) | χ7213 containing pΔcpxRA | This study |
| χ7213(pcpxRA) | χ7213 containing pcpxRA | This study |
| χ7213(pspy::lacZ) | χ7213 containing pspy::lacZ | This study |
| Plasmids | ||
| pSTNSK | pST76-K::tnsABCD; Kanr | 33 |
| pBluescript SK+ | High-copy-no. cloning vector; Ampr | Stratagene |
| pBSΔcpxRA | ΔcpxRA deletion construct in pBluescript SK+ | This study |
| pRE112 | Sucrose-sensitive (sacB1) suicide vector; Cmr | 50 |
| pΔcpxRA | ΔcpxRA deletion construct in pRE112 | This study |
| pGP-Tn7-Gm | pGP704::Tn7-Gm; Ampr Gentr | 33 |
| pcpxRA | C. rodentium DBS100 cpxRA cloned into pGP-Tn7-Gm | This study |
| pFUSE | Suicide vector, lacZYA, mob+ (RP4), ori R6K, Cmr | 34 |
| pspy::lacZ | spy promoter fusion to lacZ in pFUSE | This study |