FIG 1.

Recombinant LppQ-N′ reacts with CBPP immune sera. Immunoblots were probed with CBPP-negative bovine serum (1:100) (A), antihistidine MAb (1:4,000) (B) and CBPP-positive serum (1:100) (C). Arrows indicate the rLppQ-N′ molecule as well as the native protein in M. mycoides subsp. mycoides lysate. Recombinant histidine-tagged GlpO was used as a positive control, hence causing the positive signals in panels B and C. The high background in panel C reflects cross-reacting antibodies to E. coli proteins in unpurified rGlpO lysate.