FIG 3.
Internalization of nonmucoid and mucoid colonies by human monocytes. THP1 cells at 2 × 105 cells were incubated for 2 h with B. pseudomallei at an MOI of 1:1, after which nonadherent bacteria were killed by incubating them for 2 h with kanamycin. The bacterial count was enumerated by cell lysis and plating onto TSA. The data represent mean numbers of CFU/ml ± standard deviations for each isogenic pair from 5 B. pseudomallei isolates and are expressed as the percentages of cells internalized at 4 h compared with the number of cells in the inoculum.